Active Recombinant Canine FGF9 protein, mFc-tagged

• Cat.No.: FGF9-170C
• Product Overview: Recombinant Canine FGF9 protein(NP_002001.1)(Leu4-Ser208), fused with mFc tag, was expressed in HEK293.

Specification

• Species: Canine
• Source: HEK293
• Tag: mFc
• Protein Length: 4-208 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 5-30 ng/mL.
• Molecular Mass: The recombinant canine FGF9 comprises 441 amino acids and has a predicted molecular mass of 49.8 kDa. The apparent molecular mass of the protein is approximately 50 kDa in SDS-PAGE under reducing conditions.
• Proteinlength: Leu4-Ser208
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 75 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.