Recombinant Canine GDF15 protein, His-tagged

• Cat.No.: GDF15-1515C
• Product Overview: Recombinant Canine GDF15 protein(A0A8C0TNP6)(Ala197-Val307), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Source: E.coli
• Species: Canine
• Tag: N-His
• Protein length: Ala197-Val307
• Form: Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Canine GDF15, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 13.12 kDa. The protein migrates to 15-20 kDa under reduced (R) condition and 23-26 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE.
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).