Active Native Clostridium perfringens Phospholipase C, Type I

• Cat.No.: Neuraminidase-012C
• Product Overview: Type I, protein, 20-40%

Specification

• Species: Clostridium perfringens (C. welchii)
• Description: We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles.
• Form: Lyophilized powder
• CAS Number: 9001-86-9
• Enzyme Commission number: 3.1.4.3 (BRENDA, IUBMB)
• EC Number: 232-638-2
• MDL number: MFCD00131895
• UNSPSC Code: 12352204
• NACRES: NA.54
• Biochem/physiol Actions: PLC hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol. This enzyme also hydrolyzes the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids. Phospholipase C is induced by thrombin and platelet-activating factor, forming 1,2-diacylglycerol and phosphatidic acid.
  Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.
• Bio-activity: 10-50 units/mg protein
• Unit Definition: One unit will liberate 1.0 μmole of water soluble organic phosphorus from egg yolk L-α-phosphatidylcholine per min at pH 7.3 at 37 centigrade.
• Applications: Phospholipase C (PLC) has been used to study adrenoceptor-mediated transmembrane signaling. It is also used to degrade inositol-containing phospholipids. Furthermore, it has been used to study 9E3 gene activation
• Storage: At -20 centigrade

Gene Information

• Official Symbol: Neuraminidase
• Synonyms: Lecithinase C; Lipophosphodiesterase I; PC-PLC; Phosphatidylcholine cholinephosphohydrolase