Active Native Clostridium perfringens Choloylglycine Hydrolase

• Cat.No.: Neuraminidase-011C
• Product Overview: Protein, ≥50% biuret

Specification

• Species: Clostridium perfringens (C. welchii)
• Form: Lyophilized powder
• CAS Number: 37289-07-9
• Enzyme Commission number: 3.5.1.24 (BRENDA, IUBMB)
• MDL number: MFCD00130789
• UNSPSC Code: 12352204
• NACRES: NA.54
• Biochem/physiol Actions: The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg2+, Cu2+, and Cd2+) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.
• Bio-activity: ≥100 units/mg protein
• Unit Definition: One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 centigrade.
• Applications: The enzyme has been used in the analysis of bile samples in various studies.
• Storage: At -20 centigrade
• Storage Buffer: Partially purified lyophilized powder containing buffer salts and stabilizer
• Shipping: Wet ice

Gene Information

• Official Symbol: Neuraminidase
• Synonyms: 3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase