Acitve Recombinant Rat ErbB4 protein(Met1-Pro651), His-tagged

• Cat.No.: ErbB4-1253R
• Product Overview: Recombinant Rat ERBB4 (AAQ77349.1)(Met1-Pro651) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Met1-Pro651
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human NRG1 Beta 1 Fc at 2 μg/ml (100 μl/well) can bind Rat ErbB4 His, the EC50 of Rat ErbB4 His is 12-60 ng/mL.
• Molecular Mass: The recombinant Rat ERBB4 comprises 637 amino acids and predicts a molecular mass of 71.3 kDa. The apparent molecular mass of the recombinant protein is approximately 75-95 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Erbb4 v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) [ Rattus norvegicus ]
• Official Symbol: ErbB4
• Synonyms: ERBB4; v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian); receptor tyrosine-protein kinase erbB-4; c-erbB-4; receptor tyrosine kinase; proto-oncogene-like protein c-ErbB-4
• Gene ID: 59323
• mRNA Refseq: NM_021687
• Protein Refseq: NP_067719