Active Recombinant Human ERBB4 protein, hFc&His-tagged

• Cat.No.: ERBB4-2651H
• Product Overview: Active Recombinant Human ErbB-4/HER4 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln26-Arg649) of human Her4/ErbB4 (Accession #NP_005226.1) fused with an Fc, 6×His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: Gln26-Arg649
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human NRG1-beta1 at 2 μg/mL (100 μL/well) can bind Recombinant Human HER4/ErbB4, the EC50 of HER4/ErbB4 is 20.99 ng/mL.
  2.Measured by its binding ability in a functional ELISA.Immobilized Human ErbB4/Her4 Antibody at 1μg/mL (25 μL/well) can bind Human ErbB4/Her4 with a linear range of 0.46-14.6 ng/mL.
• Molecular Mass: 130-140 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: > 97% by SDS-PAGE.
• Storage: Store the lyophilized protein at -20°C to -80 °C for long term.
  After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Gene Information

• Gene Name: ERBB4 v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) [ Homo sapiens ]
• Official Symbol: ERBB4
• Synonyms: ERBB4; v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian); v erb a avian erythroblastic leukemia viral oncogene homolog like 4; receptor tyrosine-protein kinase erbB-4; proto-oncogene-like protein c-ErbB-4; tyrosine kinase-type cell surface receptor HER4; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; HER4; p180erbB4; MGC138404
• Gene ID: 2066
• mRNA Refseq: NM_001042599
• Protein Refseq: NP_001036064
• MIM: 600543
• UniProt ID: Q15303