Acitve Recombinant Human CD3E & CD3G protein(Met1-Asp126 & Met1-Ser116), Fc-Flag&mFc-tagged
Cat.No. : | CD3E & CD3G-1327H |
Product Overview : | Recombinant Human CD3E (NP_000724.1)(Met1-Asp126) was fused with the C-terminal FLAG-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; Recombinant Human CD3G (NP_000064.1)(Met1-Ser116) was fused the Fc region of Mouse IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Human CD3E/CD3G heterodimer was purified and expressed in HEK293. |
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Species : | Human |
Source : | HEK293 |
Tag : | Fc&Flag&mFc |
Protein Length : | Met1-Asp126 & Met1-Ser116 |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Measured by its ability to bind OKT3-mIgG2a in a functional ELISA. |
Molecular Mass : | The recombinant heterodimer of Human CD3E/CD3G comprises 681 (353+328) amino acids and has a calculated molecular mass of 76.9 (39.8+37.1) KDa. As a result of glycosylation, the apparent molecular mass of Human CD3E/CD3G heterodimer is approximately 42-47 KDa in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 95 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name | CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ] |
Official Symbol | CD3E; CD3G |
Synonyms | CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683; |
Gene ID | 916 |
mRNA Refseq | NM_000733 |
Protein Refseq | NP_000724 |
UniProt ID | P07766 |
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Not For Human Consumption!
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