Active Recombinant Mouse CD3E & CD3G protein, Fc-His &Fc-Flag-tagged
Cat.No. : | CD3E & CD3G-3034M |
Product Overview : | Recombinant Mouse CD3E&CD3G protein(Asp 23-Asp 108 (CD3E) & Gln 23-Ser 116 (CD3G)), fused with Fc-His and Fc-Flag tag, was expressed in HEK293. |
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Species : | Mouse |
Source : | HEK293 |
Tag : | Fc&Flag&His |
Protein Length : | Asp 23-Asp 108 (CD3E) & Gln 23-Ser 116 (CD3G) |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag at 1 μg/mL (100 μL/well) can bind Biotinylated CD3e Monoclonal Antibody (Clone : 145-2C11) with a linear range of 0.2-6 ng/mL.Immobilized CD3E Monoclonal Antibody (Clone : 145-2C11) at 1 μg/mL (100 μL/well) can bind Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag with a linear range of 0.01-0.25 μg/mL. |
Molecular Mass : | Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 41.5 kDa (CD3E) and 41.6 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. As a result of glycosylation, the protein migrates as 48-55 kDa under reducing (R) condition, and 100 kDa under non-reducing (NR) condition (SDS-PAGE). |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS. |
Storage : | For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name | Cd3e CD3 antigen, epsilon polypeptide [ Mus musculus ] |
Official Symbol | CD3E |
Synonyms | CD3E; CD3 antigen, epsilon polypeptide; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3; T3e; AI504783; CD3epsilon; |
Gene ID | 12501 |
mRNA Refseq | NM_007648 |
Protein Refseq | NP_031674 |
◆ Recombinant Proteins | ||
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CD3E & CD3G-3037C | Active Recombinant Cynomolgus CD3E & CD3G protein, His-Avi&Flag-tagged, Biotinylated | +Inquiry |
CD3E & CD3G-342H | Active Recombinant Human CD3E & CD3G protein, His & Fc & Flag-tagged | +Inquiry |
CD3E & CD3G-3038M | Active Recombinant Mouse CD3E & CD3G protein, His &Flag-tagged | +Inquiry |
◆ Cell & Tissue Lysates | ||
CD3E & CD3G-761HCL | Recombinant Human CD3E & CD3G cell lysate | +Inquiry |
Not For Human Consumption!
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