Active Recombinant Mouse CD3E & CD3G protein, Fc-His &Fc-Flag-tagged

• Cat.No.: CD3E & CD3G-3034M
• Product Overview: Recombinant Mouse CD3E&CD3G protein(Asp 23-Asp 108 (CD3E) & Gln 23-Ser 116 (CD3G)), fused with Fc-His and Fc-Flag tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc&Flag&His
• Protein Length: Asp 23-Asp 108 (CD3E) & Gln 23-Ser 116 (CD3G)
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag at 1 μg/mL (100 μL/well) can bind Biotinylated CD3e Monoclonal Antibody (Clone : 145-2C11) with a linear range of 0.2-6 ng/mL.Immobilized CD3E Monoclonal Antibody (Clone : 145-2C11) at 1 μg/mL (100 μL/well) can bind Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag with a linear range of 0.01-0.25 μg/mL.
• Molecular Mass: Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 41.5 kDa (CD3E) and 41.6 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. As a result of glycosylation, the protein migrates as 48-55 kDa under reducing (R) condition, and 100 kDa under non-reducing (NR) condition (SDS-PAGE).
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd3e CD3 antigen, epsilon polypeptide [ Mus musculus ]
• Official Symbol: CD3E
• Synonyms: CD3E; CD3 antigen, epsilon polypeptide; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3; T3e; AI504783; CD3epsilon
• Gene ID: 12501
• mRNA Refseq: NM_007648
• Protein Refseq: NP_031674