Recombinant Human CD3E & CD3G protein, Fc-tagged

• Cat.No.: CD3E & CD3G-146H
• Product Overview: Recombinant human CD3E (NP_000724.1) (Met1-Asp126) was fused with the Fc region of Llama IgG2b at the C-terminus, constructed the plasmid 1; Recombinant human CD3G (NP_000064.1) (Met1-Ser113) was fused with the Fc region of Llama IgG2b at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3E/CD3G heterodimer was purified.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-126;1-113 a.a.
• Predicted N Terminal: Asp 23 & Gln 23
• Form: Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
• Molecular Mass: The recombinant human CD3E/CD3G consists of 696 (353+343) amino acids and has a calculated molecular mass of 78.4 (39.7+38.7) KDa.
• Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Official Symbol: CD3E; CD3G
• Synonyms: CD3E & CD3G Heterodimer; IMD18; T3E; TCRE