Active Recombinant Human CD3E & CD3G protein, His-Avi-tagged, Biotinylated
Cat.No. : | CD3E & CD3G-34H |
Product Overview : | Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # NP_000724.1(CD3E) & AAI13831(CD3G)). |
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Species : | Human |
Source : | HEK293 |
Tag : | Avi&His |
Protein Length : | Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
Bio-activity : | Immobilized Mouse Anti-Human CD3 (SP34-2) at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free with a linear range of 0.016-0.25 μg/mL. |
Molecular Mass : | The protein has a calculated MW of 18.6 kDa (CD3E) and 14.7 kDa (CD3G). Subunit CD3E is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G contains no tag at the C-terminus. The reducing (R) heterodimer protein migrates as 22 kDa and 24-30 kDa due to glycosylation. |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20 centigrade to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name | CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ] |
Official Symbol | CD3E |
Synonyms | CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683; |
Gene ID | 916 |
mRNA Refseq | NM_000733 |
Protein Refseq | NP_000724 |
UniProt ID | P07766 |
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Not For Human Consumption!
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