Active Recombinant Human CD3E & CD3G protein, His-Avi-tagged, Biotinylated

Cat.No. : CD3E & CD3G-34H
Product Overview : Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # NP_000724.1(CD3E) & AAI13831(CD3G)).
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Species : Human
Source : HEK293
Tag : Avi&His
Protein Length : Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G)
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Bio-activity : Immobilized Mouse Anti-Human CD3 (SP34-2) at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free with a linear range of 0.016-0.25 μg/mL.
Molecular Mass : The protein has a calculated MW of 18.6 kDa (CD3E) and 14.7 kDa (CD3G). Subunit CD3E is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G contains no tag at the C-terminus. The reducing (R) heterodimer protein migrates as 22 kDa and 24-30 kDa due to glycosylation.
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Gene Name CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]
Official Symbol CD3E
Synonyms CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683;
Gene ID 916
mRNA Refseq NM_000733
Protein Refseq NP_000724
UniProt ID P07766

Not For Human Consumption!

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