Ubiquitin CoIP Agarose

• Cat.No.: Ubiquitin-01A
• Product Overview: Immunoprecipitation of ubiquitin and ubiquitinylated proteins with anti-Ubiquitin Nanobody conjugated to agarose beads
  • Fast, reliable & efficient one-stepImmunoprecipitation
  • Ready-to-use
  • No heavy & light antibody chains
  • Stable under harsh washing conditions
  • Suitable for downstream mass spec analysis
  • Works in samples from: mammals, plants, yeast, etc.

Specification

• Host: Camelid
• Specificity/Target: Binds to monomeric ubiquitin, ubiquitin chains, and ubiquitinylated proteins.
  Ubiquitin chains may be linked via lysines; linkages via lysines such as K11, K48 and K63 are compatible with binding by the Ubiquitin-Trap (further linkage types also likely to be compatible, but not tested).
• Tested Reactivity: Human, Mouse, Hamster, Dog, Spinach, Yeast
• Conjugate: Agarose beads; ~90 um (cross-linked 4% agarose beads)
• Elution buffer: 2xSDS-sample buffer (Lammli)
• Wash buffer compatibility: 2M NaCl, 5 mM DTT, 0 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0% SDS, 2-3 M Urea
• Type: Nanobody
• Affinity (KD): The KD for monomeric ubiquitin is 90 nM. The apparent affinity is even higher for ubiquitin chains due to avidity effects.
• Storage Buffer: 20% ethanol
• Applications: IP, CoIP
• Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!