Synthetic K48-linked Poly-ubiquitin chains (Ub2-16)

• Cat.No.: Ubiquitin-04
• Product Overview: Ubiquitin was polymerized through Lys48 using E1, UbcH5A, and the C-terminal domain of a specific HECT E3.

Specification

• Source: Synthetic
• Description: Polyubiquitin chains, linked through specific lysine residues, are useful tools for investigating the specificity and reactivity of deubiquitinylating enzymes (DUBs) and the recognition and interaction of polyubiquitin modified proteins with ubiquitin binding domain (UBDs) containing proteins.
• Form: Lyophilized
• Applications: For use in deubiquitinylating enzyme assays and polyubiquitin binding studies.
  Suggested uses:
  1. Deubiquitinylating enzyme substrates (general/linkage specific).
  2. Investigation of polyubiquitin chain recognition by and interaction with ubiquitin binding proteins.
• Notes: Due to the methods of synthesis and purification used it is likely that batch to batch variations in the exact amounts of each chain size present may occur. Although variation is most unlikely to be of any significant value, those wishing to guarantee reproducibility of results over an extended period of time are advised to purchase sufficient material for ongoing studies.
• Stability: Store unopened vial at -20 centigrade until required for use.
• Storage: Short Term Storage: -20 centigrade
  Long Term Storage: -20 centigrade
• Reconstitution: It is recommended that the product be reconstituted at a moderately high protein concentration (>1 mg/mL) and in the presence of buffer. If a more dilute reagent is required, the inclusion of an inert carrier protein (e.g. ovalbumin at 0.5-1.0 mg/mL) in the storage buffer is recommended in order to minimise loss of the reagent due to absorption.
• Handling: Avoid freeze/thaw cycles.
• Shipping: Shipped on Blue Ice

Gene Information

• Official Symbol: Ubiquitin
• Synonyms: Ubiquitin; UBB; UBC; Poly-ubiquitin; Ub2-16