Recombinant Full Length Human Ubiquitin Protein, HA tagged, Vinyl Sulfone Labeled

• Cat.No.: Ubiquitin-12HFL
• Product Overview: This product consists of a full-length human, mature ubiquitin polypeptide (amino acids 1-76), expressed in E. coli with a c-terminal warhead (Vinyl Sulfone) and an N-terminal HA-Tag.

Specification

• Description: Ubiquitin is a small (8.5 kDa) regulatory protein that has been found in almost all tissues of eukaryotic organisms. Ubiquitination can affect proteins in many ways; it can signal for their degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various linkage specificities. HA-Ubiquitin-Vinyl Sulfone is a potent, irreversible, and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic Vinyl Sulfone group. It targets three of the four major DUB families: UCH (Ubiquitin c-terminal hydrolases), USP (Ubiquitin Specific Proteases), OTU (Ovarian Tumor Proteases), and MJD (Machado-Josephin domain proteases) while JAMM metalloproteases are not inhibited.
• Source: E. coli
• Species: Human
• Tag: HA
• Protein length: 1-76aa
• Conjugation/Label: Vinyl Sulfone
• Molecular Mass: 10.1 kDa
• Purity: >97% by LCMS
• Usage: Reaction conditions will need to be optimized for each application. We recommend an initial HA-Ubiquitin Vinyl Sulfone concentration of 1-5 μM.
• Applications: HA-Ub-Vinyl Sulfone can be used for activity profiling experiments and using the HA-Tag conveniently for Immunoprecipitations in search for new or novel enzymes reacting with the c-terminal warhead.
• Notes: For Research Use Only, Not For Use In Humans.
• Storage: At -80 centigrade, Avoid multiple freeze/thaw cycles.
• Storage Buffer: 50mM MES pH 6.0, 100mM NaCl
• Concentration: 400 μM, 4.0 mg/mL