Recombinant Treponema pallidum Tpp15 Antigen, His&GST tagged, Biotinylated

• Cat.No.: Tpp15-052T
• Product Overview: The Tpp15 recombinant lipoprotein has been prepared by expressing the ORF of the corresponding gene which codifies the mature lipoprotein of 15 kDa of the spirochete Treponema pallidum and monobiotinylated in vivo.

Specification

• Species: Treponema pallidum
• Source: E.coli
• Tag: His&GST
• Description: Tpp15 is a 15 kDa major membrane inmunogen of the bacterium Treponema pallidum. It is a major immunogen during natural syphilis infection in humans. This lipoprotein has been reconised as a sensitive antigenic target for IgM responses in congenital infection.
• Tag: N-His&GST
• Conjugation/Label: Biotin
• Molecular Mass: Determined by SDS-PAGE, the protein band is between molecular markers of 45-66.2 kDa, while relative molecular mass calculated from amino acid sequence is 56,818.7 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, CE, DAS, NP, PO
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 8 and 0.15 M NaCl
• Concentration: 1.086 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Purcell BK, Swancutt MA and Radolf JD. 1990. Lipid modification of the 15 kilodalton major membrane immunogen of Treponema pallidum. Mol. Micobiol., 4:1371- 1379.
  2. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.