Recombinant Toxoplasma gondii SAG1

• Cat.No.: SAG1-597T
• Product Overview: The E.coli derived recombinant protein contains the p30 (SAG1) immunodomi1nant regions, amino acids 49-311. The fusion protein contains additional 46 amino acids derived from pET vectors.

Specification

• Species: T.gondii
• Source: E.coli
• Tag: Non
• Protein Length: 49-311 A.A.
• Description: In molecular biology, the SAG1 protein domain is an example of a group of glycosylphosphatidylinositol (GPI)-linked proteins named SRSs (SAG1 related sequence). SAG1 is found on the surface of a protozoan parasite Toxoplasma gondii. This parasite infects almost any warm-blooded vertebrate. The surface of T. gondii is coated with a family of developmentally regulated glycosylphosphatidylinositol (GPI)-linked proteins (SRSs), of which SAG1 is the prototypic member.
• Form: 50 mM Tris-HCl, 50% glycerol and 1 mM of DTT. No preservative and no stabilizer.
• Purity: Toxoplasma protein is >95.0% pure as determined by 12.0% PAGE (Coomassie staining).
• Applications: Toxoplasma antigen is suitable for ELISA and Western blots, excellent antigen for detection of Toxoplasma gondii- with minimal specificity problems.
• Notes: Immunoreactive with sera of Toxoplasma gondii-infected individuals.
• Stability: 6 months at -20°C or one year -80°C.
• Storage: Toxoplasma protein is shipped at 4°C. Upon arrival, store at -20°C.

Gene Information

• Gene Name: Q27298_TOXGO
• Official Symbol: SAG1
• Synonyms: SAG1; P30; SAG1 protein
• Gene ID: 5811
• UniProt ID: Q27298
• Function: Direct host-parasite interaction occurs at the cytoplasmic faces of the parasitophorous vacuole membrane (PVM) and the host endoplasmic reticulum (ER) membrane via GRA3 and host CAMLG association. Direct insertion of GRA3 ER retrieval motif into the host ER membrane contributes to the host ER recruitment to the PVM.