Recombinant T. gondii SAG1 Antigen, His tagged

• Cat.No.: SAG1-049T
• Product Overview: The mature anchor-less antigen p30 of the T. gondii (SAG1) has been prepared as a recombinant antigen fused to a his-tag in its C-terminal.

Specification

• Species: T.gondii
• Source: P.pastoris
• Tag: His
• Description: Major surface antigen p30 (SAG1) induces significant levels of p30 antibodies in all patients with toxoplasmosis. It has been described in the literature that this 30 kDa protein has the capacity to carry out the serological analysis of Toxoplasma infections.
• Tag: C-His
• Molecular Mass: Determined by SDS-PAGE, the protein appears in three different positions, with one band like a smear between molecular markers of 25.4 and 35 kDa, another above the marker of 45 kDa and a higher band around marker of 66.2 kDa. The relative molecular mass calculated from amino acid sequence and without glycosylation is 30,361.23 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, IE, DE, CLIA, LF
• Storage: Upon arrival, it should be aliquoted in order to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 7, 0.15 M NaCl and 0.1% polyoxyethylene (10) tridecyl ether
• Concentration: 1.26 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Santoro, F., Charif, H. and Capron, A. The immunodominant epitope of the major membrane techyzoite protein (p30) of Toxoplasma gondii. 1986, Parasite Immunol. 8:631-9.
  2. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.