Recombinant Thermus thermophilus TtCsm6 Protein, His-tagged

• Cat.No.: TtCsm6-22T
• Product Overview: Recombinant Thermus thermophilus TtCsm6 was expressed in E. coli cells using a C-terminal His tag.

Specification

• Species: Thermus thermophilus
• Source: E.coli
• Tag: His
• Description: TtCsm6 is a single-stranded RNA-specific endoribonuclease protein that consists of the N-terminal CARF domain and the C-terminal HEPN domain. The ribonuclease active site is formed by the HEPN domain dimer and it contributes to the Type-III-A CRISPR-Cas system. Upon activation of the CRISPR ancillary ribonucleases Csx1/Csm6, the HEPN active site degrades invasive RNA non-specifically. TtCsm6 is activated by the cyclic oligoadenylate second messengers that was converted from ATP by the Cas10 subunit of the Type III interference complex.
• Molecular Mass: 53 kDa
• Purity: >90%
• Stability: One year at -70 centigrade from date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 1.0 μg/μL
• Storage Buffer: Recombinant protein stored in 50 mM phosphate, pH 7.5, 300mM NaCl, 10% glycerol, 1 mM DTT.
• Shipping: Dry ice

Gene Information

• Synonyms: TtCsm6
• Protein Refseq: WP_011229148