Recombinant T. cruzi 1F8 Antigen, His&GST tagged

• Cat.No.: 1F8-002T
• Product Overview: The T. cruzi antigen 1F8 has been prepared as a recombinant antigen fused to a GST-His-tag in its N terminal. It is produced from the 1F8 gene of the T. cruzi calcium-binding protein.

Specification

• Species: T. cruzi
• Source: E.coli
• Tag: His&GST
• Description: 1F8 is the Trypanosoma cruzi calcium-binding protein. It is a 51 kDa protein that does not have any internal repetition. It is one of the antigens from this parasite which has shown a greater sensibility in the evaluations described in the literature.
• Tag: N-His&GST
• Molecular Mass: Determined by SDS-PAGE, the protein band is between molecular markers of 45-66.2 kDa, while relative molecular mass calculated from amino acid sequence is 56,838.8 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, IE, DE, CLIA, LF
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 8 and 0.15 M NaCl
• Concentration: 1.66 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. González, A., T. J., Lerner, M. Huecas, B. Sisa-Pineda, N. Nogueira, and P. M. Lizardi. Apparent generation of a segmented mRNA from two separate tándem gene families in Trypanosoma cruzi. 1985, Nucleic Acids Res. 13:5789-5803.
  2. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.