Recombinant Staphylococcus aureus Protein A protein, Biotin/His-tagged

• Cat.No.: Protein A-8149S
• Product Overview: Recombinant Staphylococcus aureus Protein A labeled with biotin and fused with the polyhistidine tag at the N-terminus consisting 299 amino acids was expressed in E. coli.

Specification

• Species: Staphylococcus aureus
• Source: E.coli
• Tag: Biotin&His
• Protein Length: 299 amino acids
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.
• Molecular Mass: DTT-reduced protein migrates as a 33.8 kDa polypeptide .
• Endotoxin: Less than 1.0 EU per μg of the Biotinylated Protein A by the LAL method.
• Purity: >95% as determined by reduced SDS-PAGE.
• Applications: The primary amine of Biotinylated Protein A was labeled by biotin for detection in ELISA, dot blot or western blot using streptavidin or avidin-conjugated probes.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).