Recombinant Sheep HBB protein, His-tagged

• Cat.No.: HBB-7867S
• Product Overview: Recombinant Sheep HBB aa. (Met1~His145) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Sheep
• Source: E.coli
• Tag: His
• Protein Length: Met1~His145
• Form: Freeze-dried powder
• Molecular Mass: 25kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >92%
• Characteristic: The isoelectric point is 6.3.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage Buffer: 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: HBB hemoglobin, beta [ Ovis aries (sheep) ]
• Official Symbol: HBB
• Synonyms: HBB; HBBA; HBBB; hemoglobin subunit beta; globin, beta
• Gene ID: 100049064
• mRNA Refseq: NM_001097648.1
• Protein Refseq: NP_001091117.1
• UniProt ID: Q1KYZ7