Recombinant SARS Plpro protein, His-tagged

• Cat.No.: PLpro-523S
• Product Overview: Recombinant SARS coronavirus (SARS-CoV) (strain WH20) papain-like protease (PLpro) (AAX16193.1) (Glu1541-Tyr1859) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Species: SARS
• Source: E.coli
• Tag: His
• Protein Length: Glu1541-Tyr1859
• Predicted N Terminal: Glu 1541
• Form: Lyophilized from sterile 50 mM Tris, 0.1 % Tween, pH 9.0. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant SARS coronavirus (SARS-CoV) (strain WH20) papain-like protease (PLpro) consists of 319 amino acids and predicts a molecular mass of 35.8 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90% as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Official Symbol: PLpro
• Synonyms: PLpro