Recombinant 2019-nCoV PLpro Protein, His-tagged

• Cat.No.: PLpro-033C
• Product Overview: Recombinant 2019-nCoV PLpro/papain-like protease, part of a large replicase polyprotein 1ab (E1564-Y1882), was expressed in E. coli cells with an N-terminal His tag.

Specification

• Species: Sars-Cov-2
• Source: E.coli
• Tag: His
• Protein Length: 1564-1882
• Description: 2019-nCoV papain-like protease (PLpro), also known as SARS-CoV-2 PLpro, is a virally encoded cysteine protease that acts on three cleavage sites of the viral polyprotein to release mature non-structural proteins (nsp) 1, 2 and 3, a process that is essential for viral replication. In addition, PLpro possesses deubiquitinating and deISGylating activities, thus negatively regulating the ubiquitin and ISG15-dependent host immune response during viral infection. PLpro also participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.
• Molecular Mass: 38 kDa
• Applications: Activity Assay, Inhibition Profile
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.2 μg/μL
• Storage Buffer: Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole, 1 mM DTT, 10% glycerol.

Gene Information

• Gene Name: ORF1ab ORF1a polyprotein;ORF1ab polyprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: ORF1ab
• Synonyms: ORF1ab; ORF1a polyprotein;ORF1ab polyprotein; ORF1a polyprotein;ORF1ab polyprotein; leader protein; nsp2; nsp3; nsp4; 3C-like proteinase; nsp6; nsp7; nsp8; nsp9; nsp10; RNA-dependent RNA polymerase; helicase; 3'-to-5' exonuclease; endoRNAse; 2'-O-ribose methyltransferase; leader protein; nsp2; nsp3; nsp4; 3C-like proteinase; nsp6; nsp7; nsp8; nsp9; nsp10; nsp11; pp1ab; translated by -1 ribosomal frameshift; pp1a
• Gene ID: 43740578
• Protein Refseq: YP_009724389