Recombinant Rhesus CD27 protein(Met1-Ile192), hFc-tagged

• Cat.No.: CD27-493R
• Product Overview: Recombinant Rhesus CD27 (XP_001104337.1) (Met1-Ile192) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Rhesus macaque
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Ile192
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Rhesus CD27-Fc at 10 μg/ml (100 μl/well) can bind biotinylated human CD70-Fc, The EC50 of biotinylated human CD70-Fc is 10.9-25.3 ng/ml.
• Molecular Mass: The recombinant rhesus comprises 413 amino acids and has a calculated molecular mass of 46.3 KDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.