Recombinant Rat Wif1 protein, His & T7-tagged

• Cat.No.: Wif1-1895R
• Product Overview: Recombinant Rat Wif1 aa. (Leu38~Ser247 (Accession # Q6IN38)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Rat
• Source: E.coli
• Tag: His&T7
• Protein Length: Leu38~Ser247
• Description: Human homolog has decreased mRNA expression in some prostate cancers and other cancers; may act as a secreted inhibitor of the Wnt signaling pathway.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 26.6kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >92%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in ddH2O.
• Isoelectric Point: 7.6

Gene Information

• Gene Name: Wif1 Wnt inhibitory factor 1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Wif1
• Synonyms: Wif1; Wnt inhibitory factor 1; WIF-1
• Gene ID: 114557
• mRNA Refseq: NM_053738.1
• Protein Refseq: NP_446190.1
• UniProt ID: Q6IN38