Recombinant Rat Vwf protein, His-tagged

• Cat.No.: Vwf-7788R
• Product Overview: Recombinant Rat Vwf aa. (Lys49~Asn230) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: Lys49~Asn230
• Description: Human homolog is a plasma glycoprotein that mediates platelet adhesion to damaged blood vessels and stabilizes blood coagulation factor VIII.
• Form: Freeze-dried powder
• Molecular Mass: 22kDa as determined by SDS-PAGE reducing conditions
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.8.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Vwf von Willebrand factor [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Vwf
• Synonyms: Vwf; von Willebrand factor
• Gene ID: 116669
• mRNA Refseq: NM_053889.1
• Protein Refseq: NP_446341.1
• UniProt ID: Q62935