Recombinant Rat Txnrd2 Protein, 490 residues

• Cat.No.: Txnrd2-15R
• Product Overview: Recombinant Rat Thioredoxin Reductase 2 (Non-tagged, no signal peptide sequence, 490 residues)

Specification

• Description: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.
• Species: Rat
• Protein length: 490 residues
• Purity: ≥ 95% as judged by gel analysis
• Unit definition: 1 U of enzyme reduces 1 μmol DTNB per min in 0.5 mL standard DTNB assay containing 2.5 mM DTNB and 0.3 mM NADPH in TE buffer (50 mM Tris-HCl, 2 mM EDTA, pH 7.5)
• Applications: DTNB assay; Insulin-coupled Thioredoxin assay; Inhibitor screening; etc.
• Notes: Centrifuge the tube briefly before opening the lid.
• Concentration: 0.2 mg/mL
• Storage Buffer: TE buffer with 50% glycerol
• Shipping: Shipped in cold pack by courier service. Store at -20 centigrade upon delivery.
• References: 1. Cheng Q, Arnér ES. (2017) Selenocysteine Insertion at a Predefined UAG Codon in a Release Factor 1 (RF1)-depleted Escherichia coli Host Strain Bypasses Species Barriers in Recombinant Selenoprotein Translation. J Biol Chem. 292:5476-5487.
  2. Arnér ES, Holmgren A. (2001) Measurement of thioredoxin and thioredoxin reductase. Curr Protoc Toxicol. Chapter 7:Unit 7.4.

Gene Information

• Gene Name: Txnrd2 thioredoxin reductase 2 [ Rattus norvegicus ]
• Official Symbol: Txnrd2
• Synonyms: TXNRD2; thioredoxin reductase 2; thioredoxin reductase 2, mitochondrial; thioredoxin reductase TR3; Tr3; Trxr2; Trxrd2; MGC93435
• Gene ID: 50551
• mRNA Refseq: NM_022584
• Protein Refseq: NP_072106