Recombinant Rat Txnrd1 Protein, 499 residues

• Cat.No.: Txnrd1-14R
• Product Overview: Recombinant Rat Thioredoxin Reductase 1 (Non-tagged, 499 residues)

Specification

• Description: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms.
• Species: Rat
• Protein length: 499 residues
• Purity: ≥ 95% as judged by gel analysis
• Unit definition: 1 U of enzyme reduces 1 μmol DTNB per min in 0.5 mL standard DTNB assay containing 2.5 mM DTNB and 0.3 mM NADPH in TE buffer (50 mM Tris-HCl, 2 mM EDTA, pH 7.5)
• Applications: DTNB assay; Insulin-coupled Thioredoxin assay; Inhibitor screening; etc.
• Notes: Centrifuge the tube briefly before opening the lid.
• Concentration: 1 mg/mL
• Storage Buffer: TE buffer with 50% glycerol
• Shipping: Shipped in cold pack by courier service. Store at -20 centigrade upon delivery.
• References: 1. Cheng Q, Arnér ES. (2017) Selenocysteine Insertion at a Predefined UAG Codon in a Release Factor 1 (RF1)-depleted Escherichia coli Host Strain Bypasses Species Barriers in Recombinant Selenoprotein Translation. J Biol Chem. 292:5476-5487.
  2. Arnér ES, Holmgren A. (2001) Measurement of thioredoxin and thioredoxin reductase. Curr Protoc Toxicol. Chapter 7:Unit 7.4.

Gene Information

• Gene Name: Txnrd1 thioredoxin reductase 1 [ Rattus norvegicus ]
• Official Symbol: Txnrd1
• Synonyms: TXNRD1; thioredoxin reductase 1; thioredoxin reductase 1, cytoplasmic; thioredoxin reductase TR1; selenoprotein oxidoreductase; NADPH-dependent thioredoxin reductase; Tr; MGC93353
• Gene ID: 58819
• mRNA Refseq: NM_031614
• Protein Refseq: NP_113802