Recombinant Rat Tek protein(Met4-Leu743), hFc-tagged

• Cat.No.: Tek-1360R
• Product Overview: Recombinant Rat TEK (NP_001099207.1)(Met 4-Leu 743) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: Fc
• Protein length: 4-743 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized S1h-3C-mANGPT2 at 10 μg/mL (100 μL/well) can bind ratTEK-Fc. The EC50 of ratTEK-Fc is 0.26-0.62μg/mL.
• Molecular Mass: The recombinant rat TEK/Fc is a disulfide-linked homodimer. The reduced monomer comprises 962 amino acids and predicts a molecular mass of 108 kDa. The apparent molecular mass of the rat TEK/Fc monomer is approximately 120-130 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Tek TEK tyrosine kinase, endothelial [ Rattus norvegicus ]
• Official Symbol: Tek
• Synonyms: TEK; TEK tyrosine kinase, endothelial; angiopoietin-1 receptor; endothelial-specific receptor tyrosine kinase; tyrosine kinases that contain immunoglobulin-like loops and epidermal growth factor-similar domains 2; Tie2; Tie-2
• Gene ID: 89804
• mRNA Refseq: NM_001105737
• Protein Refseq: NP_001099207