Recombinant Rat Sirt1 protein, His-tagged

• Cat.No.: Sirt1-1374R
• Product Overview: Recombinant Rat Sirt1 aa. (Lys238~Ala482 fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: Lys238~Ala482
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.2kDa.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.1.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Sirt1 sirtuin 1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Sirt1
• Synonyms: Sirt1; sirtuin 1; Sir2; NAD-dependent protein deacetylase sirtuin-1; NAD-dependent deacetylase sirtuin-1; Sir2alpha; sirtuin 1 ((silent mating type information regulation 2, homolog) 1
• Gene ID: 309757
• mRNA Refseq: XM_017601788.1
• Protein Refseq: XP_017457277.1