Recombinant Mouse Sirt1 Protein, Myc/DDK-tagged

• Cat.No.: Sirt1-5883M
• Product Overview: Purified recombinant protein of mouse full-length sirtuin 1 (Sirt1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene encodes a member of the sirtuin family of proteins, characterized by their deacetylase activity and proposed role in longevity. The encoded protein regulates gene expression in a wide range of cell and tissue types through its NAD+-dependent deacetylation of histones, transcription factors and transcriptional coactivators. Brain-specific overexpression of this gene has been shown to result in increased median lifespan. Viability of homozygous knockout mice for this gene varies with strain background. Homozygous knockout mice of strains that do not exhibit embryonic lethality are sterile and have a reduced lifespan. Alternative splicing results in multiple transcript variants.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 80.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Sirt1 sirtuin 1 [ Mus musculus (house mouse) ]
• Official Symbol: Sirt1
• Synonyms: Sirt1; sirtuin 1; S; Si; Sir2; Sir2a; SIR2L1; AA673258; Sir2alpha; NAD-dependent protein deacetylase sirtuin-1; NAD-dependent protein deacylase sirtuin-1; SIR2-like protein 1; regulatory protein SIR2 homolog 1; sirtuin 1 ((silent mating type information regulation 2, homolog) 1; EC 2.3.1.286
• Gene ID: 93759
• mRNA Refseq: NM_019812
• Protein Refseq: NP_062786
• UniProt ID: Q923E4