Recombinant Rat RBP4 cell lysate
Cat.No. : | RBP4-2271RCL |
Product Overview : | Rat RBP4 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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Species : | Rat |
Source : | Human Cells |
Tag : | Non |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name | Rbp4 retinol binding protein 4, plasma [ Rattus norvegicus ] |
Official Symbol | RBP4 |
Synonyms | RBP4; retinol binding protein 4, plasma; retinol-binding protein 4; RBP; PRBP; plasma retinol-binding protein; RBPA; |
Gene ID | 25703 |
mRNA Refseq | NM_013162 |
Protein Refseq | NP_037294 |
Pathway | Retinol metabolism, organism-specific biosystem; |
Function | binding; retinal binding; retinol binding; retinol binding; retinol transporter activity; retinol transporter activity; transporter activity; |
◆ Recombinant Proteins | ||
RBP4-112H | Recombinant Human RBP4, His-tagged | +Inquiry |
RBP4-31234TH | Recombinant Human RBP4 | +Inquiry |
Rbp4-448R | Recombinant Rat Rbp4, His-tagged | +Inquiry |
RBP4-442H | Recombinant Human RBP4 protein, His-tagged | +Inquiry |
RBP4-781H | Recombinant Human RBP4 Protein, Myc/DDK-tagged, C13 and N15-labeled | +Inquiry |
◆ Native Proteins | ||
RBP4-247H | Native Human Retinol Binding Protein 4 | +Inquiry |
◆ Cell & Tissue Lysates | ||
RBP4-2271RCL | Recombinant Rat RBP4 cell lysate | +Inquiry |
RBP4-1517CCL | Recombinant Canine RBP4 cell lysate | +Inquiry |
RBP4-2863HCL | Recombinant Human RBP4 cell lysate | +Inquiry |
RBP4-2304MCL | Recombinant Mouse RBP4 cell lysate | +Inquiry |
RBP4-1045CCL | Recombinant Cynomolgus RBP4 cell lysate | +Inquiry |
Not For Human Consumption!
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