Recombinant Rat Ppif, His-tagged

• Cat.No.: Ppif-109R
• Product Overview: Recombinant rat Ppif was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Description: Ppif also known as Peptidyl-prolyl cis-trans isomerase F, mitochondrial. Ppif accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis the requirement of the PPIase activity for this function is debated.
• Form: Liquid. In Phosphate buffered saline (pH7.4) containing 10% glycerol, 1mM DTT
• Molecular Mass: 21.2Da (200aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSCSDGGAR GANSSSQNPL VYLDVGADGQ PLGRVVLELK ADVVPKTAEN FRALCTGEKG FGYKGSTFHRVIPAFMCQAG DFTNHNGTGG KSIYGSRFPD ENFTLKHVGP GVLSMANAGP NTNGSQFFIC TIKTDWLDGK HVVFGHVKEG MDVVKKIESFGSKSGKTSKK IVITDCGQLS
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Ppif peptidylprolyl isomerase F [ Rattus norvegicus ]
• Official Symbol: Ppif
• Synonyms: PPIF; peptidylprolyl isomerase F; peptidyl-prolyl cis-trans isomerase F, mitochondrial; PPIase F; rotamase F; cyclophilin D; cyclophilin F; PPIase; MGC93084
• Gene ID: 282819
• mRNA Refseq: NM_172243
• Protein Refseq: NP_758443
• Pathway: Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Huntingtons disease, organism-specific biosystem; Huntingtons disease, conserved biosystem; Parkinsons disease, organism-specific biosystem; Toxoplasmosis, organism-specific biosystem; Toxoplasmosis, conserved biosystem
• Function: cyclosporin A binding; isomerase activity; peptide binding; peptidyl-prolyl cis-trans isomerase activity; protein binding