Recombinant Rat Polymerase (DNA Directed), Beta

• Cat.No.: Polb-766R
• Product Overview: Recombinant Rat DNA polymeraseβoverproduced inE. coliwith high enzymatic activity without any tag attached. The enzyme has molecular mass of 38 kDa. The amino acid sequence of the rat enzyme has 86% identity to the human homolog.

Specification

• Species: Rat
• Source: E.coli
• Tag: Non
• Description: DNA polymeraseβ is a distributive polymerase involved in base excision repair which repairs damaged DNA by excising modified bases (oxidized, methylated, deaminated etc.).
• Purity: Over 95% pure by SDS-PAGE analysis.
• Form: 1.3 mg/ml in 50mM Tris-HCl pH7.6, 0.3M KCl, 0.1mM EDTA, 1mM DTT, 20% Glycerol.
• Applications: 1) For the studies on the mechanisms of base-excision repair of DNA damage. 2) As a positive control of western blotting with anti-DNA polymerase β antibody.
• Enzyme Activity: 90 unit/ul (1 unit of the enzyme activity incorporates 1 nanomole of dNTP into acid-insoluble fraction at 37℃ in 60 min.)
• Storage: -20℃ (long period, 70℃).

Gene Information

• Gene Name: Polb polymerase (DNA directed), beta [ Rattus norvegicus ]
• Synonyms: Polb; polymerase (DNA directed), beta; DNA polymerase beta; DNA pol beta; polymerase-beta; DNA-directed DNA polymerase beta
• Gene ID: 29240
• mRNA Refseq: NM_017141
• Protein Refseq: NP_058837
• UniProt ID: P06766
• Chromosome Location: 16q12.5
• Pathway: Base excision repair
• Function: DNA binding; DNA-directed DNA polymerase activity; damaged DNA binding; enzyme binding; lyase activity; metal ion binding; microtubule binding; nucleotidyltransferase activity; protein binding; transferase activity

Reviews

09/07/2023

Efficacy: The enzyme is still active and I am currently using it in some DNA repair assays. It works very well for single nucleotide insertions in gapped DNA which is mostly what I have been using it for recently. Quality: Very happy with the quality and longevity. I think the enzyme is ~1yr old with frequent usage and it is still working fine. Overall Experience: Very happy. When we need more polB/hUNG, and potentially others, we will purchase from you again.