Recombinant Rat Nr1h4, His-tagged

• Cat.No.: Nr1h4-07R
• Product Overview: The Recombinant Rat Nr1h4, His-tagged protein was expressed in E. coli and purified by affinity chromatography in combination with FPLC columns.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Description: Farnesoid-X-receptor (FXR) was originally identified and cloned in rat as an orphan nuclear hormone receptor based on hybridization with a degenerate oligonucleotide designed from the highly conserved nuclear hormone receptor DNA binding domain. FXR functions as a heterodimer with RXR and binds to sequence elements in the promoters of target genes. The FXR/RXR heterodimer binds with highest affinity to inverted repeats separated by 1 bp (IR-1) and with low affinity to direct repeats separated by 4 and 5 bp (DR-4 and DR-5). As is the case for other nuclear hormone receptors, FXR regulates target gene activity in response to ligand. While initial studies suggested that farnesol and retinoid metabolites were likely ligands for FXR, current data support the notion that FXR is a bile acid sensor that plays an integral role in bile acid synthesis and transport.
• Form: The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol.
• Molecular Mass: 56.6 kDa.
• AA Sequence: MVMQFQGLEN PIQISLQHSH RLSGFASDRM SSKPAKGVLT EHAAGPLGQN LDLESYSPYN NVQFPQVQPQ ISSSSYYSNL GFYPQQPEDW YSPGLYELRR MPTESVYQGE TEVSEMPVTK KPRMAASSAG RIKGDELCVV CGDRASGYHY NALTCEGCKG FFRRSITKNA VYKCKNGGNC VMDMYMRRKC QDCRLRKCRE MGMLAECLLT EIQCKSKRLR KNVKQHADQT VNEDSEGRDL RQVTSTTKLC REKTELTVDQ QTLLDYIMDS YSKQRMPQEI TNKILKEEFS AEENFLILTE MATSHVQILV EFTKRLPGFQ TLDHEDQIAL LKGSAVEAMF LRSAEIFNKK LPAGHADLLE ERIRKSGISD EYITPMFSFY KSVGELKMTQ EEYALLTAIV ILSPDRQYIK DREAVEKLQE PLLDVLQKLC KIYQPENPQH FACLLGRLTE LRTFNHHHAE MLMSWRVNDH KFTPLLCEIW DVQ
• Purity: Greater than 90% as determined by SDS-PAGE.
• Storage: Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Gene Information

• Gene Name: Nr1h4 nuclear receptor subfamily 1, group H, member 4 [ Rattus norvegicus ]
• Official Symbol: Nr1h4
• Synonyms: NR1H4; nuclear receptor subfamily 1, group H, member 4; bile acid receptor; farnesol receptor HRR-1; RXR-interacting protein 14; farnesoid X activated receptor; farnesoid X-activated receptor; retinoid X receptor-interacting protein 14; Fxr; MGC94878
• Gene ID: 60351
• mRNA Refseq: NM_021745
• Protein Refseq: NP_068513
• UniProt ID: Q62735
• Chromosome Location: 7q13
• Pathway: Bile secretion, organism-specific biosystem;Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem
• Function: DNA binding; RNA polymerase II distal enhancer sequence-specific DNA binding; bile acid binding