Recombinant Rat Mylk2 protein, His & T7-tagged

• Cat.No.: Mylk2-8032R
• Product Overview: Recombinant Rat Mylk2 aa. (Ala327~Thr536 (Accession # P20689)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Rat
• Source: E.coli
• Tag: His&T7
• Protein Length: Ala327~Thr536
• Description: Kinase; phosphorylates a serine in the N-terminus of a myosin light chain.
• Form: Freeze-dried powder
• Molecular Mass: 25kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Mylk2 myosin light chain kinase 2 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Mylk2
• Synonyms: Mylk2; myosin light chain kinase 2; Klmc; Mlck; myosin light chain kinase 2, skeletal/cardiac muscle; MLCK2; myosin light chain kinase 2, skeletal muscle; myosin, light polypeptide kinase 2
• Gene ID: 117558
• mRNA Refseq: NM_057209.1
• Protein Refseq: NP_476557.1
• UniProt ID: P20689