Recombinant Rat Masp2 protein, His-tagged

• Cat.No.: Masp2-4644R
• Product Overview: Recombinant Rat Masp2 protein(A2VCV7)(Thr287-Phe685), fused with C-terminal His tag, was expressed in E.coli.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: Thr287-Phe685
• Tag: C-His
• Form: Supplied as 0.22μm filtered solution in 50mM Tris, 200mM NaCl (pH 9.0).
• Bio-activity: Immobilized Rat MASP2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 13.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 46.7 kDa. Due to Autocatalytic cleavage, the protein migrates to 15-20 kDa&25-30 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Valid for 12 months from date of receipt when stored at -80°C; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Masp2 mannan-binding lectin serine peptidase 2 [ Rattus norvegicus ]
• Official Symbol: Masp2
• Synonyms: MASP2; mannan-binding lectin serine peptidase 2; mannan-binding lectin serine protease 2; MBL-associated serine protease 2; mannose binding lectin-associated serine protease-2; mannose-binding protein-associated serine protease 2; MAp19; MASP-2
• Gene ID: 64459
• mRNA Refseq: NM_172043
• Protein Refseq: NP_742040