Recombinant Rat Kirrel3 protein(Met1-Ala523), His-tagged

• Cat.No.: Kirrel3-8741R
• Product Overview: Recombinant Rat KIRREL3 (Q09GS6) (Met1-Ala523) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 1-523 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of MS1 mouse pancreatic islet endothelial cells. When cells are added to rat KIRREL3 coated plates (15 μg/mL, 100 μL/well), > 20% will adhere specifically after 90 minutes at 37 °C.
• Molecular Mass: The recombinant rat KIRREL3 comprises 513 amino acids and predicts a molecular mass of 56.4 kDa. The apparent molecular mass of the recombinant protein is approximately 60-64 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Kirrel3 kin of IRRE like 3 (Drosophila) [ Rattus norvegicus ]
• Official Symbol: Kirrel3
• Synonyms: KIRREL3; kin of IRRE like 3 (Drosophila); kin of IRRE-like protein 3
• Gene ID: 315546
• mRNA Refseq: NM_001048215
• Protein Refseq: NP_001041680