Recombinant Rat Interleukin 5

• Cat.No.: Il5-97R
• Product Overview: Recombinant Rat Interleukin-5 produced inE.Coliis a dimeric, non-glycosylated polypeptide chain containing 113 amino acids and having a molecular mass of 13074 Dalton. The IL-5 is purified by proprietary chromatographic techniques.

Specification

• Species: Rat
• Source: E.coli
• Tag: Non
• Description: The protein encoded by this gene is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. This cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of this cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. This cytokine, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Ile-Pro-Met.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: Lyophilized from a concentrated (1 mg/ml) solution in water containing no additives.
• Solubility: It is recommended to reconstitute the lyophilized Interleikin-5 Rat in sterile 18 MΩ-cm H₂O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Specific Activity: The ED₅₀as determined by the dose-dependant prolifiration of Con A activated rat spleen cells is < 1.5 ng/ml, corresponding to a Specific Activity of 6.6 x 10⁵ IU/mg.
• Stability: Lyophilized Interleukin-5 Rat although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IL5 Rat should be stored at 4℃ between 2-7 days and for future use below -18℃. Please avoid freeze thaw cycles.

Gene Information

• Gene Name: Il5 interleukin 5 [ Rattus norvegicus ]
• Synonyms: Il5; interleukin 5;Interleukin 5 (colony-stimulating factor eosinophil); Interleukin 5 (colony-stimulating factor, eosinophil)
• Gene ID: 24497
• mRNA Refseq: NM_021834
• Protein Refseq: NP_068606
• UniProt ID: Q08125
• Chromosome Location: 10q22
• Pathway: Allograft rejection; Asthma; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Hematopoietic cell lineage; Jak-STAT signaling pathway; T cell receptor signaling pathway
• Function: cytokine activity; growth factor activity; interleukin-5 receptor binding