Active Recombinant Human IL5 Protein (116 aa)

• Cat.No.: IL5-354I
• Product Overview: Recombinant Human Interleukin-5 (rhIL-5) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 116 amino acids each. A fully biologically active molecule, rhIL-5 has a molecular mass of 26.5 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Protein Length: 116
• Description: Interleukin-5 (IL-5), produced by mast cells, T cells and eosinophils, is responsible for the activities attributed to eosinophil differentiating factor, B cell growth factor II and T cell-replacing factor (TRF). It can increase production and mobilization of eosinophils and CD34+ progenitors from the bone marrow. IL-5 plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors. IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: ED50 < 1.0 ng/mL, measured by a cell proliferation assay using TF-1 Cells, corresponding to a specific activity of > 1.0 × 10^6 units/mg.
• Molecular Mass: 26.5 kDa, observed by non-reducing SDS-PAGE.
• AA Sequence: MIPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 95% by SDS-PAGE.
• Storage: Lyophilized recombinant Human Interleukin-5 (rhIL-5) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhIL-5 should be stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against 25mM Tris, pH8.0.
• Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Gene Information

• Gene Name: IL5 interleukin 5 (colony-stimulating factor, eosinophil) [ Homo sapiens ]
• Official Symbol: IL5
• Synonyms: IL5; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; B cell differentiation factor I; EDF; eosinophil differentiation factor; IL 5; interleukin 5; T cell replacing factor; TRF; T-cell replacing factor; B-cell differentiation factor I; IL-5
• Gene ID: 3567
• mRNA Refseq: NM_000879
• Protein Refseq: NP_000870
• MIM: 147850
• UniProt ID: P05113