Recombinant Rat Hgf protein(Met1-Leu728)

• Cat.No.: Hgf-8806R
• Product Overview: Recombinant Rat HGF (NP_058713.1)(Met1-Leu728) was expressed and purified in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: Non
• Protein Length: 1-728 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using Mv-1-1u mink lung epithelial cells. The ED50 for this effect is typically 1-6 ng/mL.
• Molecular Mass: The recombinant rat HGF consists of 696 amino acids and has a predicted molecular mass of 79.3 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 92 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Hgf hepatocyte growth factor [ Rattus norvegicus ]
• Official Symbol: Hgf
• Synonyms: HGF; hepatocyte growth factor; SF; scatter factor; hepatopoeitin-A; hepatopoietin-A; HPTA
• Gene ID: 24446
• mRNA Refseq: NM_017017
• Protein Refseq: NP_058713