| Species : |
Human |
| Tag : |
Non |
| Description : |
Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. |
| Form : |
Lyophilised:Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5 mg/ml. This solution can then be diluted into otheraqueous buffers and stored at 4°C for 1 week or -20°C for future use. |
| Storage : |
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Sequences of amino acids : |
hHGF (Human Hepatocyte Growth Factor) is an 80 kDa disulfide-linked heterodimeric protein consisting of the alpha chain (463 amino acids) and the beta chain (234 amino acids):QRKRRNTIHEFKKSAKTTLIKIDPALK IKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQ CLWFPFNSMSSQVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRG KDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQ CSEVECMTCNGESYRGLMGHTESGKICQRWDHQTPHR HKFLPERYPDKGFDDNYCRNPDGGPRPWCYTLDPHTRW EYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGT VNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENY CRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQD CYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIF WEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYC PISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPS RDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPE GSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGK VTLNESEICAGAEKIGSQPCEGDYGGPLVCEQHKMR MVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILT YKVPQS |
| Sequence Similarities : |
Belongs to the peptidase S1 family. Plasminogen subfamily.Contains 4 kringle domains.Contains 1 PAN domain.Contains 1 peptidase S1 domain. |
