Recombinant Rat glycoprotein (transmembrane) nmb Protein, mIgG2a tagged

• Cat.No.: Gpnmb-10R
• Product Overview: Recombinant Rat Osteoactivin/GPNMB Protein (Lys23-Asn500) with Mouse IgG2a (Glu98-Lys330) Fc tag at C-terminus was expressed in HEK293.

Specification

• Description: Predicted to enable heparin binding activity; signaling receptor binding activity; and syndecan binding activity. Involved in bone mineralization and osteoblast differentiation. Predicted to be located in cytoplasmic vesicle. Predicted to be integral component of membrane. Predicted to be integral component of plasma membrane. Human ortholog(s) of this gene implicated in primary localized cutaneous amyloidosis 3. Orthologous to human GPNMB (glycoprotein nmb).
• Source: HEK293
• Species: Rat
• Tag: C-mIgG2a
• Protein length: 23-500aa
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Integrin alpha V beta 3 is immobilized at 2 μg/mL (100 μL/well), Recombinant Rat Osteoactivin/GPNMB Fc Chimera binds with an ED50 of 0.700-7.00 μg/mL.
• Molecular Mass: 54 kDa
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• N-terminal Sequence Analysis: Lys23
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: Gpnmb glycoprotein nmb [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Gpnmb
• Synonyms: GPNMB; glycoprotein (transmembrane) nmb; transmembrane glycoprotein NMB; osteoactivin
• Gene ID: 113955
• mRNA Refseq: NM_133298
• Protein Refseq: NP_579832
• UniProt ID: Q6P7C7