Recombinant Mouse glycoprotein (transmembrane) nmb Protein, mIgG2a tagged

• Cat.No.: Gpnmb-08M
• Product Overview: Recombinant Mouse Osteoactivin/GPNMB Protein (Lys23 - Asn502) with mouse IgG2A (Glu98 - Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Specification

• Description: Enables heparin binding activity and integrin binding activity. Involved in several processes, including negative regulation of tumor necrosis factor production; positive regulation of ERK1 and ERK2 cascade; and positive regulation of protein phosphorylation. Acts upstream of or within cell adhesion. Located in cytoplasmic vesicle. Is integral component of plasma membrane. Is expressed in several structures, including alimentary system; brain; eye; heart ventricle; and mesenchyme derived from neural crest. Used to study pigment dispersion syndrome. Human ortholog(s) of this gene implicated in primary localized cutaneous amyloidosis 3. Orthologous to human GPNMB (glycoprotein nmb).
• Source: NS0
• Species: Mouse
• Tag: C-mIgG2a
• Protein length: 23-502aa
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells. When 0.25 μg/mL (100 μL/well) of rmOsteoactivin/Fc Chimera is immobilized on goat anti-mouse Fc Chimera antibody coated wells, it will support > 30% adhesion of added SVEC4-10 cells (1×10^5 cells/well).
• Molecular Mass: 80.3 kDa
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: > 90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• N-terminal Sequence Analysis: Lys23
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.

Gene Information

• Gene Name: Gpnmb glycoprotein (transmembrane) nmb [ Mus musculus (house mouse) ]
• Official Symbol: Gpnmb
• Synonyms: GPNMB; glycoprotein (transmembrane) nmb; transmembrane glycoprotein NMB; osteoactivin; iris pigment dispersion; dendritic cell-associated transmembrane protein; dendritic cell-associated heparin sulfate proteoglycan-dependent integrin ligand; ipd; Dchil; DC-HIL
• Gene ID: 93695
• mRNA Refseq: NM_053110
• Protein Refseq: NP_444340
• UniProt ID: Q99P91