Recombinant Human glycoprotein (transmembrane) nmb Protein, Fc&Avi tagged, Biotin Labeled

• Cat.No.: GPNMB-11H
• Product Overview: Recombinant Human Osteoactivin/GPNMB Protein (Lys23-Asn486) with Human IgG1 (Pro100-Lys330) Fc tag and Avi-tag at C-terminus was expressed in Human embryonic kidney cell.

Specification

• Description: The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: C-Fc&Avi
• Protein length: 23-486aa
• Conjugation/Label: Biotin
• Form: Disulfide-linked homodimer
  Biotinylated via Avi-tag
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Human Integrin alpha V beta 3 is immobilized at 2 μg/mL (100 μL/well), Biotinylated Recombinant Human Osteoactivin/GPNMB Fc Chimera Avi-tag binds with an ED50 of 1.00-12.0 μg/mL.
• Molecular Mass: 80 kDa
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: > 85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• N-terminal Sequence Analysis: Lys23
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: GPNMB glycoprotein nmb [ Homo sapiens (human) ]
• Official Symbol: GPNMB
• Synonyms: GPNMB; glycoprotein (transmembrane) nmb; transmembrane glycoprotein NMB; glycoprotein NMB; glycoprotein nmb like protein; HGFIN; NMB; osteoactivin; transmembrane glycoprotein; glycoprotein nmb-like protein; transmembrane glycoprotein HGFIN
• Gene ID: 10457
• mRNA Refseq: NM_001005340
• Protein Refseq: NP_002501
• MIM: 604368
• UniProt ID: Q14956