Recombinant Rat Fto, His-tagged

• Cat.No.: Fto-273R
• Product Overview: Rat FTO (aa 2-502) is fused at the N-terminus to a His-tag.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: 2-502 a.a.
• Description: FTO (Fat mass-and obesity-associated gene) is the responsible gene for mouse ‘fused toes’ mutation. An association between FTO genotype and type 2 diabetes has been confirmed. The presence of the FTO rs9939609 A-allele was found to be positively correlated with other symptoms of the metabolic syndrome, including higher fasting insulin, glucose, triglycerides, and lower HDL-cholesterol.
• Form: Liquid. 0.2μm-filtered solution in 50mM TRIS-Cl, pH 8.2, containing 150mM NaCl, 1mM DTT.
• Molecular Mass: ~55kDa (SDS-PAGE)
• Purity: Purified using Ni-NTA column and FPLC.
• Stability: Working aliquots are stable for up to 3 months when stored at -20°C.
• Storage: Short Term Storage: +4°C; Long Term Storage: -20°C. After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.

Gene Information

• Gene Name: Fto fat mass and obesity associated [ Rattus norvegicus ]
• Official Symbol: Fto
• Synonyms: FTO; fat mass and obesity associated; alpha-ketoglutarate-dependent dioxygenase FTO; protein fto; fat mass and obesity-associated protein; RGD1305121
• Gene ID: 291905
• mRNA Refseq: NM_001039713
• Protein Refseq: NP_001034802
• Function: DNA-N1-methyladenine dioxygenase activity; DNA-N1-methyladenine dioxygenase activity; ferrous iron binding; ferrous iron binding; metal ion binding; molecular_function; oxidative DNA demethylase activity; oxidative DNA demethylase activity; oxidative RNA demethylase activity; oxidative RNA demethylase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen