Active Recombinant Full Length Human FTO Protein, FLAG tagged

• Cat.No.: FTO-05HFL
• Product Overview: Recombinant FTO protein was expressed in a Baculovirus expression system as the full length protein (accession number XP_011521615.1) with an N-terminal FLAG-Tag.

Specification

• Species: Human
• Source: Insect cells
• Tag: Flag
• Protein Length: 1-505 aa
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Storage Buffer: 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-1000.2 mM TCEP.
• Applications: Recombinant FTO protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling. 3 μM m6A ssDNA oligos (DNA sequence: 5'-GTTGCCTGTTCGTGTTGG/m6A/ CTTGCCTGT-3') were incubated with FTO protein in reaction buffer including 50 mM HEPES pH 7.5, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2·6H2O and 1 mM TCEP for 2 hours at room temperature. Dot-Blot was used for activity detection.
• Concentration: 0.3 μg/μL

Gene Information

• Official Symbol: FTO
• Synonyms: FTO; fat mass and obesity associated; alpha-ketoglutarate-dependent dioxygenase FTO; KIAA1752; MGC5149; protein fto; fat mass and obesity-associated protein
• Gene ID: 79068
• mRNA Refseq: NM_001080432
• Protein Refseq: NP_001073901
• MIM: 610966
• UniProt ID: Q9C0B1