Recombinant Rat Cxcl5 protein

• Cat.No.: Cxcl5-629R
• Product Overview: Recombinant Rat Cxcl5 protein was expressed in Escherichia coli.

Specification

• Species: Rat
• Source: E.coli
• Tag: Non
• Protein Length: 93
• Description: CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. The expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-γ. Recombinant rat CXCL5 contains 93 amino acids which is a single, non-glycosylated polypeptide chain. Rat CXCL5 shares 57 % amino acid sequence identity with human CXCL5.
• Form: Lyophilized from a 0.2μm filtered concentrated solution in 2 × PBS, pH 7.4.
• Bio-activity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10-100 ng/ml.
• Molecular Mass: Approximately 10.1 kDa, a single, non-glycosylated polypeptide chain containing 93 amino acids.
• AA Sequence: APFSAMVATELRCVCLTLAPRINPKMIANLEVIPAGPHCPKVEVIAKLKNQKDNVCLDPQAPLIKKVIQKILGSENKKTKRNALALVRSASTQ
• Endotoxin: Less than 1 EU/μg of rRtLIX/CXCL5 as determined by LAL method.
• Purity: >97% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: Cxcl5
• Official Symbol: Cxcl5
• Synonyms: CXCL5; chemokine (C-X-C motif) ligand 5; C-X-C motif chemokine 5; cytokine LIX; CXC chemokine LIX; small-inducible cytokine B5; chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); Cxcl6
• Gene ID: 60665
• mRNA Refseq: NM_022214
• Protein Refseq: NP_071550
• UniProt ID: P97885