Active Recombinant Rat Cxcl5 Protein (88 aa)

• Cat.No.: Cxcl5-227C
• Product Overview: Recombinant rat LIX/CXCL5 (88aa) produced in CHO cells is a polypeptide chain containing 88 amino acids. A fully biologically active molecule, rrLIX/CXCL5 (88aa) has a molecular mass of 9.6 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.

Specification

• Species: Rat
• Source: CHO
• Protein Length: 88
• Description: LPSinduced CXC chemokine (LIX), also known as C-X-C motif chemokine 5(CXCL5), is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. Rat LIX cDNA encodes a 130 aa residue precursor with a predicted 37 aa residue signal peptide and a 93 aa residue mature protein. Among human CXC chemokines, rat LIX is most closely related to human GCP-2 and ENA-78.LIX can signal through the CXCR2 receptor.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: The EC50 value of rat LIX/CXCL5 (88aa) on Ca^2+ mobilization assay in CHO-K1/Gα15/rCXCR2 cells (human Gα15 and rat CXCR2 stably expressed in CHO-K1 cells) is less than 3 μg/mL.
• Molecular Mass: 9.6 kDa, observed by reducing SDS-PAGE.
• AA Sequence: APFSAMVATELRCVCLTLAPRINPKMIANLEVIPAGPHCPKVEVIAKLKNQKDNVCLDPQAPLIKKVIQKILGSENKKTKRNALALVR
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 98% as analyzed by SDS-PAGE.
• Storage: Lyophilized recombinant Rat LIX/CXCL5(88aa) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rat LIX/CXCL5(88aa) should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against PBS.
• Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Gene Information

• Gene Name: Cxcl5 chemokine (C-X-C motif) ligand 5 [ Rattus norvegicus ]
• Official Symbol: Cxcl5
• Synonyms: CXCL5; chemokine (C-X-C motif) ligand 5; C-X-C motif chemokine 5; cytokine LIX; CXC chemokine LIX; small-inducible cytokine B5; chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); Cxcl6
• Gene ID: 60665
• mRNA Refseq: NM_022214
• Protein Refseq: NP_071550
• UniProt ID: P97885