Recombinant Rat CD38 protein(Trp45-Val303), His-tagged

• Cat.No.: Cd38-8743R
• Product Overview: Recombinant Rat CD38 (Q64244) (Trp45-Val303) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: His
• Protein length: 45-303 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDPribose. The specific activity is >100, 000pmols/min/ug.
• Molecular Mass: The recombinant rat CD38 comprises 270 amino acids and predicts a molecular mass of 31.2 kDa. The apparent molecular mass of the recombinant protein is approximately 42 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  Characterization of CD38 in the Major Cell Types of the Heart: Endothelial Cells Highly Express CD38 with Activation by Hypoxia/Reoxygenation Depleting NAD(P)H. (2017)
  Characterization of CD38 in the Major Cell Types of the Heart: Endothelial Cells Highly Express CD38 with Activation by Hypoxia/Reoxygenation Depleting NAD(P)H. (2017)

Gene Information

• Gene Name: Cd38 CD38 molecule [ Rattus norvegicus ]
• Official Symbol: CD38
• Synonyms: CD38; CD38 molecule; ADP-ribosyl cyclase 1; CD38H; cADPr hydrolase 1; cyclic ADP-ribose hydrolase 1; CD38 antigen (ADP-ribosyl cyclase / cyclic ADP-ribose hydrolase)
• Gene ID: 25668
• mRNA Refseq: NM_013127
• Protein Refseq: NP_037259