Recombinant Rat C3 protein, His-tagged

• Cat.No.: C3-7845R
• Product Overview: Recombinant Rat C3 aa. (Asp966~Trp1313 (Accession # P01026)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: Putative complement component C3; likely involved in innate immune response.
• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 40.3kDa
• Protein length: Asp966~Trp1313
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.4.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: C3 complement C3 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: C3
• Synonyms: C3; complement C3; complement component 3
• Gene ID: 24232
• mRNA Refseq: NM_016994.2
• Protein Refseq: NP_058690.2
• UniProt ID: P01026

Reviews

02/23/2022

Expression efficiency is high. In our experiments, we found that the target protein can be synthesized quickly and effectively.

01/26/2022

Activity was not significantly different from the native protein, as expected.

03/23/2021

High purity, no obvious impurities.

Q&As

Have proteins been found to interact with C3 proteins?

Yes, a variety of proteins that interact with C3 proteins, including complement receptors, regulatory proteins, inflammatory factors, etc., have been discovered, and they are jointly involved in the regulation and functional execution of C3 proteins.

What is the relationship between C3 protein and infectious diseases?

C3 protein plays an important role in infectious diseases. It can play an anti-infection role by activating the complement system, directly killing pathogens or enhancing the ability of immune cells to destroy pathogens.

How is the research progress of C3 protein at present?

Research on C3 protein is going on. At present, it mainly focuses on the analysis of its function and regulatory mechanism, the identification of gene mutations associated with diseases, and the interaction with other complement proteins and immune cells, which provides a theoretical basis for the further development of diagnosis and treatment methods of related diseases.

What is the use of C3 protein in laboratory diagnostics?

C3 protein can be used as an aid in the diagnosis of certain immune-related diseases. Measuring the level of C3 protein in serum can reflect the activity of the immune system and the degree of inflammation.

What are the effects of post-translational modifications of C3 protein on its function?

Post-translational modifications of the C3 protein can affect its function. Phosphorylation, cleavage, methylation and other modifications can regulate the activity, stability and interaction ability of C3 proteins.

Have any drug treatments associated with C3 protein been identified?

Yes, some C3 protein-related drug treatments are under development, including drugs that regulate the degree of C3 protein activation, drugs that inhibit the overactivation of C3 protein, etc.